Figure: Glycosaminoglycan biosynthetic process, linker formation (Mouse)¶
This figure depicts the glycosaminoglycan (GAG) biosynthetic process, specifically the linker formation pathway in mouse (Mus musculus) cells. The process occurs in the Golgi apparatus (GO:0005794) and involves multiple enzymes that sequentially add sugar moieties to form the tetrasaccharide linker between proteoglycans and their GAG chains.
The pathway begins with UDP-xylose synthesis by Uxs1 (UDP-glucuronate decarboxylase) and transport by Slc35b4. Xylosyltransferases (Xylt1 and Xylt2) then transfer xylose to serine residues on core proteins. This is followed by the sequential addition of two galactose units by B4galt7 and B3galt6, respectively. Finally, one of three glucuronosyltransferases (B3gat1, B3gat2, or B3gat3) adds glucuronic acid to complete the linker structure.
Each enzyme is represented by a green circle with its gene name and function, while substrates are shown as yellow rectangles. The process flows from left to right and top to bottom, with dashed lines indicating enzyme-substrate interactions. All enzymes are localized to the Golgi apparatus as specified in the GO-CAM model.
Feedback from AI on figure:
{"feedback":"This drawing effectively illustrates the glycosaminoglycan biosynthetic process focusing on linker formation in mouse cells. The diagram maintains a clear, professional style suitable for journals like Cell or Nature with excellent visual organization. The sequential steps are clearly numbered and labeled, and the use of color coding (green for enzymes, yellow for substrates, blue for transporters) helps distinguish different components. Each enzyme is properly annotated with both its gene name and function, along with relevant GO terms. The pathway flow is intuitive, following a logical progression with clear directional arrows. The inclusion of brief descriptions for each enzymatic function directly within the enzyme circles adds clarity without cluttering the diagram. The Golgi apparatus localization is properly depicted as the cellular context for this process, staying faithful to the GO-CAM model content.","necessary_changes":null,"optional_changes":null}