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Figure: Insulin processing 1 (Mouse)

id: gomodel:62f58d8800001500

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This diagram illustrates the insulin processing pathway in mouse pancreatic β cells as described in the GO-CAM model (gomodel:62f58d8800001500). The pathway shows three key stages:

  1. In the endoplasmic reticulum (ER), insulin (INS2) undergoes proper folding and disulfide bond formation. ERO1B provides disulfide oxidoreductase activity, P4HB facilitates protein-disulfide reduction, and HSP90B1 acts as a protein folding chaperone.

  2. After transport to the Golgi apparatus, zinc is loaded into insulin granules via the zinc transporter SLC30A5 located in the Golgi membrane.

  3. In secretory granules, insulin undergoes proteolytic processing by PCSK1 (serine-type endopeptidase), PCSK2 (serine-type endopeptidase), and CPE (metallocarboxypeptidase). SLC30A8 transports zinc into the secretory granules, where zinc ions (blue dots) facilitate insulin hexamer formation. SLC30A3 may also contribute to zinc transport (shown as a dashed line indicating an implicit interaction).

The diagram depicts the sequential cellular compartments (ER, Golgi, secretory granules) where insulin processing occurs before mature insulin is secreted from the β cell.

Feedback from AI on figure:

{"feedback":"This diagram effectively illustrates the insulin processing pathway in mouse pancreatic beta cells, capturing all key elements from the GO-CAM model. The sequential process flows clearly from the ER through the Golgi to secretory granules, with each compartment distinctly visualized.\n\nThe illustration includes:\n1. Clear representation of all gene products from the GO-CAM (ERO1B, P4HB, HSP90B1, INS2, SLC30A5, PCSK1, PCSK2, CPE, SLC30A8, SLC30A3)\n2. Well-defined cellular compartments with appropriate coloring and labels\n3. Accurate representation of molecular functions (disulfide bond formation, protein folding, zinc transport, proteolytic processing)\n4. Intuitive flow of the pathway with directional arrows\n5. Helpful annotations indicating the nature of interactions\n\nThe legend is comprehensive and explains the biological context of the diagram, making it suitable for inclusion in a scientific journal. The style appropriately balances detail and clarity, as would be expected in a Cell or Nature figure.","necessary_changes":null,"optional_changes":null}