Figure: USP44-mediated deubiquitination of CDC20 controlling anaphase initiation (Human)¶
This figure illustrates the molecular pathway controlling anaphase initiation through USP44-mediated deubiquitination of CDC20 in human cells.
The pathway depicts interactions between key proteins: 1. USP44 (deubiquitinase) - removes ubiquitin from CDC20, inhibiting anaphase initiation 2. CDC20 (APC/C activator) - when deubiquitinated, prevents APC/C activation 3. UBE2C (E2 ubiquitin-conjugating enzyme) - adds ubiquitin to CDC20, promoting APC/C activation 4. ANAPC11 (E3 ubiquitin ligase) - component of the APC/C complex that promotes anaphase initiation
The diagram shows two opposing regulatory mechanisms: - Negative regulation: USP44 deubiquitinates CDC20 in the mitotic spindle, preventing anaphase - Positive regulation: UBE2C ubiquitinates CDC20 in the APC/C complex, promoting anaphase
This precise balance of ubiquitination and deubiquitination activities serves as a critical checkpoint controlling the metaphase-to-anaphase transition during mitosis in human cells.
Feedback from AI on figure:
{"feedback":"This pathway diagram effectively illustrates the USP44-mediated deubiquitination of CDC20 in controlling anaphase initiation. The diagram successfully captures the key molecular interactions and regulatory mechanisms in a visually appealing and scientifically accurate manner suitable for publication in journals like Cell or Nature.","necessary_changes":null,"optional_changes":"The diagram could be further enhanced by adding specific substrates of ANAPC11 that are targeted for degradation during anaphase initiation, such as securin or cyclin B, if those details were available in the source GO-CAM data."}