Figure: Isg12a K6-ubiquitination by TRIM21 leading to pyroptosis (Human)¶
This figure illustrates the molecular pathway of Isg12a K6-ubiquitination by TRIM21 leading to pyroptosis in human cells. TRIM21 (UniProtKB:P19474) in the cytoplasm exhibits ubiquitin protein ligase activity (GO:0061630), catalyzing K6-linked ubiquitination (GO:0085020) of IFI27/Isg12a (UniProtKB:P40305) located in the mitochondrion. IFI27/Isg12a, functioning as a molecular adaptor (GO:0060090), positively regulates CASP3 (UniProtKB:P42574) in the cytoplasm. CASP3, with its cysteine-type endopeptidase activity (GO:0004197), subsequently activates GSDME (UniProtKB:O60443) at the plasma membrane. Activated GSDME exhibits wide pore channel activity (GO:0022829), forming pores in the plasma membrane that ultimately result in cell content release and pyroptotic inflammatory response (GO:0070269).
Feedback from AI on figure:
{"feedback":"This diagram effectively illustrates the Isg12a K6-ubiquitination pathway by TRIM21 leading to pyroptosis, faithfully representing the GO-CAM model in a visually clear manner suitable for publication in high-impact journals. The pathway components are accurately positioned in their appropriate cellular compartments (cytoplasm, mitochondrion, and plasma membrane), with clearly labeled proteins and their functional activities. The sequential regulation from TRIM21 to IFI27/Isg12a to CASP3 to GSDME is well-represented with directional arrows and appropriate labels indicating the type of regulation. The pore formation and cell content release resulting from GSDME activation effectively illustrates the outcome of the pathway. The color-coding enhances visual distinction between different proteins, and the comprehensive legend provides easy reference for understanding the diagram components. Text sizes are appropriate for readability, and the overall layout presents a logical flow from the initiating ubiquitination event to the culminating pyroptotic response.","necessary_changes":null,"optional_changes":null}