Figure: Ubiquitination of RagA/RRAGA by RNF152 inhibits the mTORC1 signaling (Human)¶
The pathway illustrates how RNF152 ubiquitinates RagA/RRAGA to inhibit mTORC1 signaling in response to amino acid starvation.
Key components: - RNF152: E3 ubiquitin ligase located on the lysosomal membrane - RagA/RRAGA: Small GTPase that switches between GDP-bound (inactive) and GTP-bound (active) states - GATOR1: GTPase-activating protein complex for RagA - mTORC1: Mechanistic target of rapamycin complex 1, a master regulator of cell growth
Signaling pathway: 1. Amino acid starvation triggers the pathway 2. RNF152 catalyzes K63-linked ubiquitination of RagA 3. GATOR1 functions as a GTPase-activating protein (GAP) for RagA 4. This inhibits GTP binding to RagA, keeping it in GDP-bound form 5. GTP-bound RagA is required to recruit mTORC1 to the lysosomal membrane 6. Without GTP-bound RagA, mTORC1 signaling is inhibited
This mechanism provides a regulatory checkpoint that prevents mTORC1 activation during nutrient limitation, ensuring cellular resources are conserved when amino acids are scarce.
Feedback from AI on figure:
{"feedback":"The diagram effectively illustrates the ubiquitination pathway of RagA/RRAGA by RNF152 and its inhibitory effect on mTORC1 signaling. The drawing presents a clear scientific visualization appropriate for a high-quality journal publication, with well-organized spatial relationships between components on the lysosomal membrane.\n\nKey strengths:\n1. The diagram uses appropriate visual hierarchies with clear labels and consistent styling\n2. The color-coding helps distinguish different proteins and complexes\n3. The numbered annotations guide the viewer through the signaling cascade\n4. The legend provides clear identification of all components\n5. The arrows and inhibition symbols effectively show the relationships between components\n\nThe visual styling effectively conveys the scientific information while maintaining the aesthetic standards expected in publications like Cell or Nature.","necessary_changes":null,"optional_changes":null}