Figure: USP9X-TRIM32 modulation of IQCB1 during G2-M (Human)¶
Color Code: - Pink (red border): USP9X with molecular sequestering activity - Green (green border): MARCHF7 and TRIM32 with ubiquitin ligase activity - Blue (blue border): IQCB1 with protein-macromolecule adaptor activity - Light blue area: Centrosome (GO:0005813) - Gray dashed line: Implied cilium structure (not explicitly in GO-CAM) - Gray clock: Cell cycle indicator (implied, not in source)
Key GO Terms: - GO:0005737 - Cytoplasm - GO:0005813 - Centrosome - GO:0060271 - Cilium Assembly - GO:1902018 - Negative Regulation of Cilium Assembly - GO:0140313 - Molecular Sequestering Activity (USP9X) - GO:0061630 - Ubiquitin Protein Ligase Activity (TRIM32, MARCHF7) - GO:0030674 - Protein-Macromolecule Adaptor Activity (IQCB1)
The diagram illustrates how USP9X in the cytoplasm sequesters MARCHF7, while both MARCHF7 and TRIM32 at the centrosome negatively regulate cilium assembly through their ubiquitin ligase activity targeting IQCB1, which functions as an adaptor in cilium assembly.
Feedback from AI on figure:
{"feedback":"This pathway diagram clearly illustrates the USP9X-TRIM32 modulation of IQCB1 during G2-M phase in human cells. The cellular compartmentalization, protein interactions, and regulatory relationships are all accurately represented with appropriate visual elements. The diagram effectively communicates how USP9X in the cytoplasm exhibits molecular sequestering activity affecting MARCHF7 at the centrosome, while both MARCHF7 and TRIM32 negatively regulate cilium assembly through their ubiquitin ligase activity targeting IQCB1. The color coding and spatial organization help distinguish different components and their functions, making this appropriate for publication in high-impact journals like Cell or Nature.","necessary_changes":null,"optional_changes":null}