Figure: SQSTM1 mediates retention of KEAP1 into p62 bodies (Human).¶
This diagram illustrates how SQSTM1 (p62) mediates the retention of KEAP1 into p62 bodies in human cells.
The pathway begins with cellular stress (GO:0033554) activating ULK1 kinase, which phosphorylates SQSTM1/p62 (GO:0004674). Once phosphorylated, SQSTM1/p62 performs two key functions:
- It acts as a molecular condensate scaffold (GO:0140693) to form p62 bodies, which are intracellular membraneless organelles (GO:0043232).
- It sequesters KEAP1 protein (GO:0140311) and retains it within these p62 bodies.
When KEAP1 is sequestered within p62 bodies, its normal function as a ubiquitin-ligase adaptor (GO:1990756) in the ubiquitin-dependent protein catabolic process (GO:0006511) is inhibited. This sequestration prevents KEAP1 from targeting NFE2L2/Nrf2 for degradation (though NFE2L2 is not explicitly mentioned in the source GO-CAM).
Color coding in the diagram: ULK1 (blue), SQSTM1/p62 (green), KEAP1 (pink), and p62 bodies (yellow). Different arrow styles represent distinct types of interactions: solid lines for direct effects, dashed lines for formation processes, and dash-dotted lines for inhibited functions.
Feedback from AI on figure:
{"feedback":"The diagram effectively illustrates the SQSTM1-mediated pathway for KEAP1 retention in p62 bodies, presenting a clear visual representation suitable for publication in a scientific journal. The visual elements employ appropriate scientific conventions while maintaining artistic quality.\n\nKey strengths:\n- Clear depiction of the cellular stress response triggering ULK1 phosphorylation of SQSTM1/p62\n- Effective visualization of p62 body formation and KEAP1 sequestration\n- Appropriate use of color coding to distinguish different proteins and structures\n- Well-labeled pathway steps with corresponding GO terms\n- Informative legend that clarifies the diagram components\n- Good text contrast and readability\n- Differentiated arrow styles to indicate different types of interactions\n\nThe diagram successfully captures the key components and relationships described in the GO-CAM model while presenting them in a publication-quality figure that would be appropriate for a high-impact journal.","necessary_changes":null,"optional_changes":null}