Figure: Atg7-mediated autophagosome assembly via ATM-CHK2-TRIM32 axis¶
The diagram illustrates the Atg7-mediated autophagosome assembly pathway through the ATM-CHK2-TRIM32 axis. The primary components include:
- ATM: Serine/threonine kinase activated during cellular stress response
- CHK2: Downstream kinase activated by ATM phosphorylation
- TRIM32: E3 ubiquitin ligase that mediates K63-linked ubiquitination of ATG7
- ATG7: E1-like enzyme with dual activities (Atg12 and Atg8 activating)
- MAP1LC3B: Ubiquitin-like protein that binds to phosphatidylethanolamine (PE) on the autophagosome membrane
The pathway proceeds from stress response signaling in the cytoplasm, through the phagophore assembly site, to culminate in autophagosome assembly. ATG7's two distinct enzymatic activities at the phagophore assembly site are crucial for the lipidation process that leads to MAP1LC3B binding to the autophagosome membrane.
Feedback from AI on figure:
{"feedback":"The diagram effectively illustrates the Atg7-mediated autophagosome assembly pathway via the ATM-CHK2-TRIM32 axis. The cellular compartmentalization is clearly depicted, showing how the signaling begins in the cytoplasm with ATM activation and proceeds to the phagophore assembly site where ATG7 functions, ultimately leading to autophagosome formation.\n\nThe use of distinct colors and shapes helps differentiate between protein types (kinases, ligases, etc.) and their functions. The comprehensive legend enhances understanding of the different components. The diagram maintains an appropriate balance between scientific accuracy and visual clarity, making it suitable for publication in high-impact journals like Cell or Nature.\n\nThe improved text size and weight for protein labels and interaction descriptions enhances readability. Additional annotations for specific processes (K63-linked ubiquitination, lipidation process) provide important mechanistic context without overwhelming the figure.","necessary_changes":null,"optional_changes":null}