Figure: Phosphorylation of NBN regulates DNA repair choice at telomes (Human).¶
This diagram illustrates how phosphorylation of NBN (Nibrin) regulates DNA repair choice at telomeres in human cells. The pathway shows two main branches:
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Protection Pathway (Left): CDK2 (Cyclin-Dependent Kinase 2) phosphorylates NBN, which then uses its chromatin-protein adaptor activity to interact with TERF2 (Telomeric Repeat-Binding Factor 2). This leads to protection from non-homologous end joining (NHEJ) at telomeres.
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Repair Pathway (Right): PPP1CA (Protein Phosphatase 1 Catalytic Subunit Alpha) dephosphorylates NBN, which then interacts with TERF2 through its adaptor activity. This promotes alternative non-homologous end joining (Alt-NHEJ) DNA repair.
Both pathways involve TERF2 binding to telomeric DNA and contribute to telomere maintenance in response to DNA damage. Phosphorylation status of NBN acts as a molecular switch determining which DNA repair pathway is used at telomeres.
Feedback from AI on figure:
{"feedback":"The diagram effectively illustrates the phosphorylation of NBN and its role in regulating DNA repair choice at telomeres. The visual representation includes all key components from the GO-CAM model while maintaining clarity and scientific accuracy.\n\nThe diagram's strengths include:\n- Clear color-coding to distinguish between the protection and repair pathways\n- Appropriate use of shapes to represent different proteins and processes\n- Inclusion of all relevant GO terms and UniProt identifiers\n- Well-structured layout showing the nuclear context and telomeric region\n- Informative legend explaining abbreviations and terminology\n- Enhanced readability with appropriate font sizes and line weights\n\nThe diagram successfully captures the molecular mechanisms described in the GO-CAM model, showing how CDK2 phosphorylates NBN leading to protection from NHEJ, while PPP1CA dephosphorylates NBN leading to alternative NHEJ repair, with both pathways involving TERF2 interaction with telomeric DNA.","necessary_changes":null,"optional_changes":null}