Figure: CARD9 regulation by TRIM62/USP15 (Human)¶
The illustration depicts the CARD9 regulation pathway by TRIM62 and USP15 in human cells, based on the provided GO-CAM model.
Key components: - CLEC7A: A pattern recognition receptor at the plasma membrane that initiates the signaling cascade - CARD9: A signaling adaptor protein that plays a central role in this pathway - BCL10 and MALT1: Form a signaling complex with CARD9 to promote NF-κB activation - TRIM62: An E3 ubiquitin ligase that adds K27-linked ubiquitin to CARD9, inhibiting its function - USP15: A deubiquitinase that removes ubiquitin from CARD9, restoring its function
The pathway shows how CLEC7A activation triggers CARD9 signaling, which recruits BCL10 and MALT1 to promote NF-κB activation. This process is regulated by a balance between TRIM62-mediated ubiquitination (inhibitory) and USP15-mediated deubiquitination (activating) of CARD9.
The red dashed line represents the impaired signaling from ubiquitinated CARD9, which is not explicitly stated in the GO-CAM but is a logical inference based on the regulatory mechanism described.
Feedback from AI on figure:
{"feedback":"This SVG illustration effectively captures the CARD9 regulation pathway described in the GO-CAM model. The diagram follows scientific journal figure standards with clear organization, appropriate use of colors and shapes to differentiate protein types, and concise annotations including GO terms. The cellular compartmentalization (plasma membrane and cytoplasm) provides important spatial context, and the arrows with varied styles effectively convey different types of relationships between components. The regulatory balance between TRIM62 and USP15 is particularly well-represented, showing how these proteins oppositely influence CARD9 activity through ubiquitination and deubiquitination. The figure successfully communicates both the molecular details and the overall signaling flow from receptor activation to NF-κB regulation.","necessary_changes":null,"optional_changes":"The illustration effectively presents the CARD9 regulation pathway in a clear, visually appealing manner suitable for a scientific journal. The compartmentalization clearly shows where each protein functions (plasma membrane vs. cytoplasm), and the use of different shapes and colors makes it easy to distinguish between protein types. The detailed annotations, including GO terms, provide valuable context for understanding the molecular functions involved. The arrows clearly indicate the direction of signal flow, and the increased line thickness improves visibility. The legend is comprehensive and the text size has been adjusted for better readability. The addition of the \"impaired signaling\" pathway (marked in red) is a logical inference that helps complete the narrative of the regulatory mechanism, while being properly identified as not explicitly stated in the source GO-CAM."}