Figure: RNA capping (GO:0036260)¶
The diagram illustrates the RNA capping pathway in Schizosaccharomyces pombe (fission yeast). This process, which occurs in the nucleus, involves the sequential addition of a 7-methylguanosine (m7G) cap to the 5' end of nascent RNA transcripts.
Key components and process steps: 1. RNA Polymerase II Complex - Initiates transcription 2. rpb1 (chromatin-protein adaptor) - Activates mcs6 kinase 3. mcs6 (CTD S5 kinase) - Phosphorylates the C-terminal domain (CTD) of RNA Pol II 4. pct1 (mRNA 5'-triphosphate monophosphatase) - Removes a phosphate from the 5' end of nascent RNA 5. ceg1 (mRNA guanylyltransferase) - Adds a GTP cap to the dephosphorylated RNA 6. pcm1 (cap methyltransferase) - Methylates the GTP cap to form the m7G cap
The capping process shown here is critical for mRNA stability, export from the nucleus, and recognition by the translation machinery. The m7G cap protects the mRNA from degradation by exonucleases and is required for efficient translation initiation. This figure accurately represents the genetic model in the GO-CAM, showing the relationships between gene products and their molecular functions in the RNA capping pathway.
Feedback from AI on figure:
{"feedback":"The diagram effectively communicates the RNA capping pathway in S. pombe with excellent clarity and scientific accuracy. The sequential nature of the process is well-represented through the left-to-right flow, with clear connections between each enzyme and its role in the pathway. The color-coding system is intuitive and properly explained in the legend, making it easy to distinguish different types of enzymes (kinases, transferases, etc.).\n\nThe visual representation of RNA molecules with their evolving caps is particularly effective, showing the progressive changes to the RNA 5' end throughout the capping process. The nuclear localization is appropriately depicted, and the relationships between proteins (like rpb1 activating mcs6) are clearly shown with labeled arrows.\n\nThe addition of a process description provides important context about the biological significance of RNA capping, enhancing the educational value of the figure. Overall, this diagram would be suitable for inclusion in a high-quality scientific journal, as it balances technical accuracy with visual clarity.","necessary_changes":null,"optional_changes":null}