Figure: GPI anchor biosynthetic process (GO:0006506)¶
The diagram illustrates the GPI anchor biosynthetic process (GO:0006506) in Schizosaccharomyces pombe. The pathway begins with phosphatidylinositol (PI) synthesis by the pis1 enzyme and progresses through sequential steps of enzymatic modifications.
The GPI-GnT complex (gpi1, gpi2, gpi3, gpi15) initiates the pathway by adding N-acetylglucosamine to PI. Next, gpi12 performs deacetylation, followed by acylation by gwt1.
Three sequential mannose additions are performed by gpi14, gpi18, and a series of mannosyltransferases (its8, gpi10, smp3). Ethanolamine phosphate groups are attached by gpi7 and gpi13 enzymes.
GPI lipid remodeling occurs via bst1 (PI deacylase), gup1 (O-acyltransferase), and cwh43. The completed GPI anchor is transferred to proteins by the GPI-transamidase complex (containing gpi8, gpi16, gab1, gpi17, and gaa1).
Regulatory proteins pga1, pig25, and pbn1 modulate the pathway activity. The DPM complex (dpm1, dpm2, dpm3) in the cytoplasm contributes essential components for mannose addition.
This entire process occurs in the endoplasmic reticulum, with synthesis beginning on the cytoplasmic face and anchor attachment happening in the ER lumen.
Feedback from AI on figure:
{"feedback":"The diagram effectively captures the complex GPI anchor biosynthetic process in S. pombe as described in the GO-CAM model. The illustration presents a clear, scientifically accurate representation of the stepwise pathway with appropriate subcellular localization and enzyme relationships.\n\nStrengths:\n- Comprehensive inclusion of all proteins from the GO-CAM model\n- Clear visual distinction between enzymes, complexes, and regulators\n- Logical flow of the pathway with directional arrows showing sequence\n- Helpful descriptions of enzyme functions\n- Inclusion of both the biochemical pathway and final GPI anchor structure\n- Professional appearance suitable for journal publication\n\nThe diagram follows the style of figures found in Cell or Nature, with clear organization, appropriate use of color coding, and comprehensive labeling. All key elements of the biosynthetic process are represented, from the initial phosphatidylinositol synthesis through the various modification steps to the final attachment of the GPI anchor to proteins.","necessary_changes":null,"optional_changes":null}