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Based on my review of GO-CAM model 66187e4700001744 and the relevant documentation, I'll now provide a comprehensive review of the model.

Review of GO-CAM Model: gomodel:66187e4700001744

Model Overview

Title: "meiotic cohesion protection in anaphase I (GO:1990813)"
Taxon: Fission yeast (NCBITaxon:4896)
Status: Production
Link: https://bioregistry.io/go.model:66187e4700001744

This model represents the biological process of meiotic centromeric cohesion protection during anaphase I in fission yeast, which ensures that sister chromatids remain attached at centromeres throughout the first meiotic division.

Model Strengths

  1. Biological accuracy: The model accurately captures the key components of the meiotic cohesion protection pathway, including Moa1, Plo1, Spc7, Shugoshin (Sgo1), PP2A phosphatase, and Rec8. The interactions represented align with current scientific understanding as evidenced in the literature.

  2. Well-connected activities: The model uses appropriate causal relations (RO:0002629 - directly positively regulates) between activities, showing clear functional connections between proteins in the pathway.

  3. Appropriate molecular functions: The molecular functions assigned to each gene product (e.g., kinetochore adaptor activity, protein serine/threonine kinase activity) are accurate and supported by evidence.

  4. Cellular context: The model correctly places activities in their appropriate cellular locations (kinetochore, condensed chromosome centromeric region).

  5. Evidence attribution: Each activity and relationship is supported by appropriate evidence codes and PMID references.

Areas for Improvement

  1. Missing occurs_in relation for some activities: Several activities in the model lack an "occurs_in" relation, including:
  2. Activity gomodel:66187e4700001744/66187e4700001974 (PomBase:SPBC16H5.07c, protein phosphatase activity)
  3. Activity gomodel:66187e4700001744/66187e4700001997 (PR:000050512, molecular adaptor activity)

  4. Activity gomodel:66187e4700001744/66187e4700001845 (PomBase:SPCC1322.12c, kinase activity)

  5. Incomplete evidence for some causal associations: Some causal associations lack evidence, such as:

  6. The relationship between PR:000050512 (Rec8) adaptor activity and Sgo1 activity

  7. One of the causal associations from SPAC664.01c (Swi6) to SPBP35G2.03c (Sgo1)

  8. Missing key input molecules: For the molecular adaptor activity (GO:0060090) enabled by PR:000050512 (Rec8), there are no "has input" relations defined. According to the literature and GO-CAM best practices, adaptors should specify the molecules they bring together through "has input" relations.

  9. Potential simplification opportunity: The model shows two nearly identical causal associations from SPAC664.01c (Swi6) to SPBP35G2.03c (Sgo1), which appears redundant and could be consolidated.

  10. Incomplete part_of relations: Some activities are missing "part_of" relations to the overall biological process (GO:1990813), particularly for PR:000027566 and PR:000027557.

Suggestions for Improvement

  1. Add occurs_in relations: Add appropriate cellular component locations for all activities that are currently missing this information.

  2. Add evidence for causal associations: Provide evidence for the currently unsupported causal associations, particularly for the Rec8 adaptor activity relationships.

  3. Specify input molecules for adaptor activities: For the molecular adaptor activity (GO:0060090) enabled by PR:000050512 (Rec8), add "has input" relations to specify which molecules this adaptor brings together.

  4. Consolidate redundant relations: Review and consolidate the duplicate causal associations from SPAC664.01c (Swi6) to SPBP35G2.03c (Sgo1).

  5. Complete part_of relations: Ensure all activities have appropriate "part_of" relations to the overall biological process.

  6. Enhance molecular clarity: According to the literature, phosphorylation of Rec8 by Plo1 at S450 is a key event that promotes PP2A action. This specific mechanism could be more explicitly represented in the model.

Scientific Consistency

The model accurately represents the current understanding of meiotic cohesion protection as described in the literature. The paper by Ma et al. (2021) supports the key relationships depicted in the model, particularly the role of Moa1-Plo1 in phosphorylating Rec8, which enhances Sgo1-PP2A activity to protect centromeric cohesion.

The model captures the hierarchical regulation where: 1. Moa1 recruits Plo1 to kinetochores 2. Plo1 phosphorylates Spc7 and Rec8 3. Phosphorylated Spc7 recruits Bub1 4. Bub1 phosphorylates histone H2A 5. Phosphorylated H2A recruits Sgo1 (Shugoshin) 6. Sgo1 brings PP2A to centromeres 7. PP2A dephosphorylates Rec8, preventing its cleavage by separase

Conclusion

This GO-CAM model provides a good representation of the meiotic cohesion protection pathway in fission yeast. With the suggested improvements, particularly regarding adaptor inputs and evidence for causal associations, the model would more comprehensively represent this important biological process and better adhere to GO-CAM best practices.