Class: LibraryPreparationProtocol

URI: hca:LibraryPreparationProtocol

Attributes

Own

• ➞describedBy _1..1
  • Description: The URL reference to the schema.
  • Range: String
• ➞schema_version _0..1
  • Description: The version number of the schema in major.minor.patch format.
  • Range: String
• ➞schema_type _1..1
  • Description: The type of the metadata schema entity.
  • Range: LibraryPreparationProtocol_schema_type_options
• ➞provenance _0..1
  • Description: Provenance information provided by the system.
  • Range: Provenance
• ➞protocol_core _1..1
  • Description: Core protocol-level information.
  • Range: ProtocolCore
• ➞cell_barcode _0..1
  • Description: Information about cell identifier barcodes.
  • Range: Barcode
• ➞spatial_barcode _0..1
  • Description: Information about spatial barcodes.
  • Range: Barcode
• ➞input_nucleic_acid_molecule _1..1
  • Description: Starting nucleic acid molecule isolated for sequencing.
  • Range: BiologicalMacromoleculeOntology
• ➞nucleic_acid_source _1..1
  • Description: Source cells or organelles from which nucleic acid molecules were collected.
  • Range: LibraryPreparationProtocol_nucleic_acid_source_options
• ➞library_construction_method _1..1
  • Description: The general method for sequencing library construction.
  • Range: LibraryConstructionOntology
• ➞library_construction_kit _0..1
  • Description: Name of kit used to construct the sequencing library.
  • Range: PurchasedReagents
• ➞nucleic_acid_conversion_kit _0..1
  • Description: Name of kit used to convert RNA to DNA for sequencing.
  • Range: PurchasedReagents
• ➞end_bias _1..1
  • Description: The type of tag or end bias the library has.
  • Range: LibraryPreparationProtocol_end_bias_options
• ➞primer _0..1
  • Description: Primer used for cDNA synthesis from RNA.
  • Range: LibraryPreparationProtocol_primer_options
• ➞strand _1..1
  • Description: Library strandedness.
  • Range: LibraryPreparationProtocol_strand_options
• ➞spike_in_kit _0..1
  • Description: Information about a spike-in kit.
  • Range: PurchasedReagents
• ➞spike_in_dilution _0..1
  • Description: Dilution of spike-in.
  • Range: Integer
• ➞umi_barcode _0..1
  • Description: Information about unique molecular identifier (UMI) barcodes.
  • Range: Barcode
• ➞library_preamplification_method _0..1
  • Description: The method used to amplify RNA prior to adaptor ligation.
  • Range: LibraryAmplificationOntology
• ➞cdna_library_amplification_method _0..1
  • Description: The method used to amplify cDNA library prior to sequencing.
  • Range: LibraryAmplificationOntology
• ➞nominal_length _0..1
  • Description: Average (insert) size of the fragments being sequenced.
  • Range: Integer
• ➞nominal_sdev _0..1
  • Description: Standard deviation of the (insert) size of the fragments being sequenced.
  • Range: Integer